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Image Search Results
Journal: Molecules and Cells
Article Title: Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection Formats
doi: 10.14348/molcells.2016.0167
Figure Lengend Snippet: Gap capacitance impedimetric sensor. (A) Layout of gap capacitance impedance sensor chip. Top and bottom electrode are bonded by parylene layer. The parylene layer also works as fluidic channel in-between the electrode. Layout of whole chip and real chip is shown below. (B) Aptamer immobilization . Thiol modified, cy3 tagged Ec3(31) was immobilized on the Au electrode. Various concentrations were used for coating the electrode. Immobilization efficiency was monitored by fluorescence measurement. (C) Nyquist plot from impedance analysis of E. coli. and microscopic visualization of bacteria on the chip. Bound bacteria was stained with Sybr Gold and visualized under fluorescent microscopy. Imaginary and actual impedance was plotted on the Y and X axis respectively. The high frequency area is being shown here. At higher frequency (over 1 kHz) resistance increases in proportion to the number of bacteria in solution.
Article Snippet: After 1 h of incubation, the chip was washed thoroughly and the bound bacteria were stained with Sybr Gold and visualized under
Techniques: Modification, Fluorescence, Bacteria, Staining, Microscopy